A recombinant phage active against Shewanella sp. was assembled and a recombinant live phage was recovered with the native Shewanella host species with a reporting system from the genomic DNA data of Yushan virus.
A DNA biosensor based on impedimetry was developed for the rapid detection of P. monodon.
An IoT-based LED light chamber system for antibacterial photodynamic inactivation (aPDI) was designed and fabricated.
The cytotoxicity of ZnO-Nanoparticles was evaluated on fish cell lines such as fantail goldfish fin (FtGF) cell line and spleen cell lines from Oscar fish (OS). Both fish cell lines exhibited a higher IC50 value for ZnO nanoparticles than other human and murine cell lines.
Polyethylene glycol (PEG) coated CuO-Nanoparticles were checked for antibacterial and cytotoxicity activity and found that PEG coating had no impact on antibacterial activity, but it notably reduced cytotoxicity.
To provide a reliable and universally applicable approach for biofilm categorisation, capable of unifying the results across studies, a new standardized formula for the bio-film classification across different wavelengths was developed by adjusting the categorisation threshold using the extinction coefficient ratio (Rλ) i.e., ODc adjusted = ODCλ * Rλ. Here ODC is Optical Density Control
The Chitosan Oligo-Saccharide (COS) antibacterial activity was improved at par with the low molecular weight chitosan while adding the ZnO-Nanoparticles (ZnO-NP). Owing to the water-soluble in nature addition to the COS-ZnO-NP composite, it can be used in various biomedical applications.
A minimum inhibitory concentration assay is more suitable to the criteria to select ZnO-Nanoparticle for application related to control of the microorganisms, followed by SEM, DLS and λ max. The hierarchy for comparing the activity of better nanoparticles is as follows: MIC>MBC>SEM>DLS> λmax> FTIR.
A study on the prevalence of V. parahaemolyticus in retail freshwater fish was conducted using 100 samples, and it revealed an overall prevalence rate of 51%. This indicates that a notable percentage of freshwater fish were contaminated with V. parahaemolyticus.
Vibrio vulnificus isolates of seafood origin were screened for the resistant profile with 12 antibiotics and found that 93.3% were resistant to Cefuroxime and 80% were resistant to both Cefotaxime and Cefactor.
Two strains, Bacillus pumilus and B. subtilis, were isolated from the marine environment and identified to have biofilm-inhibiting capabilities, indicating their potential for use as probiotic strains.
A total of 35 shrimp samples were screened for Vibrio mimicus, revealing a prevalence rate of 5.71%.
Antibiotic susceptibility testing was conducted on V. mimicus isolated from shrimp farms, revealing high resistance to ampicillin, penicillin, cefotaxime, and cefepime.
S. agalactiae (n=6) resistant to cefotaxime, chloramphenicol, levofloxacin and tetracycline were isolated from the aquaculture farms.
Evaluations of the virulence, biofilm-forming, and biocide resistance potential of A. baumannii (n=11) were carried out in aquaculture settings. Two new sequence types (STs), ST1685 and ST3041, based on the Oxford scheme were found.
Whole-genome sequences (WGS) of a K. pneumoniae isolate was analyzed for antibiotic resistance genes (ARGs) and sequence types (ST) and revealed that an uncommon serotype of K. pneumoniae O3b with the high-risk clone ST37.
Three numbers of K. oxytoca WGS were submitted to NCBI: Accession no: JBGREA000000000, JBGREB000000000 & JBGREC000000000.
24 ESBL-producing P. aeruginosa were isolated from the aquaculture farm settings and evaluated for the phenotypic and genotypic characterization of AMR.31.06% of them were MDR and had a Multiple Antibiotic Resistance (MAR) index of 0.36 to 0.4.
Maintenance of cell lines Fantail Goldfish Gill (FtGG) and Oscar spleen (OS) cell lines using Leibovitz’s -15 (L-15) medium supplemented with 15% FBS at 28 °C for the isolation of aquatic viruses.
Macroplastics in aquatic environments were found to carry pathogens and antimicrobial resistance genes.
A total 49 Vibrio spp. isolates from the gut and 37 isolates from the hepatopancreas of white-gut affected shrimp were tested for antibiotic susceptibility. Vibrio spp. isolated from shrimp hepatopancreas and AST were evaluated
Whole genome data of 5 MRSA analysis revealed that the MRSA belonged to Sequence type ST6, ST6, ST8, ST39 and STNew. For diagnostic kit development, the virulence genes and AMR determinants in the genome were characterized.
SXT integrase gene of conjugative transposable element was detected in five Vibrio species.
3 (1.17%.) L. monocytogenes were recovered from the Lethrinus sp out of the 85 fishes screened.
Waste-to-wealth product launched for commercial use Bacillus based microbial consortium for household waste composting CIFT MicroBoost, and the product of composting, CIFT BioBoost for plant nutrition.
